Procedures to separate motile, normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent
normal
embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU), Swim-up from semen (SO), Continuous Percoll Gradients Ⅰ (PⅠC), Discontinuous Percoll Gradients Ⅰ (PⅠD), Continuous
Percoll
Gradients Ⅱ (PⅡC), Discontinuous Percoll Gradients Ⅱ (PⅡD), SpermPrep (SFC), Wang's tube (WT), Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate (%), morphologically
normal
& motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability.
A PⅡD proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the PⅡD has advantages as compared with other methods in terms of recovery rate, enhancement of motility and normal morphology. And
a
LTC
seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and PⅡD for separation of morphologically normal & motile sperm and acrosome reacted sperm may
be
a
useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.
|